Gene | Replicate | Total edits designed | Number of edits observed | Fraction covered (%)a | Editing efficiency (%)b |
---|---|---|---|---|---|

lysP | I | 260 | 59 | 22.7 | 1.9 |

lysP | II | 260 | 65 | 25.0 | 1.6 |

lysC | I | 380 | 132 | 34.7 | 2.7 |

lysC | II | 380 | 161 | 42.4 | 1.8 |

dapF | I | 320 | 103 | 32.2 | 2.2 |

dapF | II | 320 | 130 | 40.6 | 2.0 |

lysR | I | 820 | 358 | 43.7 | 2.8 |

lysR | II | 820 | 433 | 52.8 | 3.7 |

argP | I | 560 | 265 | 47.3 | 2.9 |

argP | II | 560 | 345 | 61.6 | 2.7 |

↵a “Fraction covered” is calculated by dividing the “Number of edits observed” by the “Total edits designed”.

↵b Editing efficiency is an estimation that takes in consideration the fraction of the total library represented by the sequenced region, according to the equation /(

*w*/16,300), with “Eff” being the estimated editing efficiency, “edits” being the number of sequencing reads that mapped to a genomic edit in the targeted window, “total” being the total number of sequencing reads, and “*w*” being the “Total edits designed”. The full list of all these values can be found in Dataset EV4.