Table 1. Deep sequencing of selected genomic regions to confirm editing
GeneReplicateTotal edits designedNumber of edits observedFraction covered (%)aEditing efficiency (%)b
lysPI2605922.71.9
lysPII2606525.01.6
lysCI38013234.72.7
lysCII38016142.41.8
dapFI32010332.22.2
dapFII32013040.62.0
lysRI82035843.72.8
lysRII82043352.83.7
argPI56026547.32.9
argPII56034561.62.7
  • a “Fraction covered” is calculated by dividing the “Number of edits observed” by the “Total edits designed”.

  • b Editing efficiency is an estimation that takes in consideration the fraction of the total library represented by the sequenced region, according to the equation Embedded Image/(w/16,300), with “Eff” being the estimated editing efficiency, “edits” being the number of sequencing reads that mapped to a genomic edit in the targeted window, “total” being the total number of sequencing reads, and “w” being the “Total edits designed”. The full list of all these values can be found in Dataset EV4.