Table 1. Global parameters A and B, which describe the baseline response of the cellular system, were computed from fitness functions of Escherichia coli BW25113 in TET, DOX, and MCN using equation (1)
MaxMinAverageLS fitSD
TET
A2.681.92.292.160.39
B1.021.371.201.150.18
DOX
A4.844.184.514.550.33
B1.321.461.391.340.07
MCN
A6.676.256.466.440.21
B2.032.362.202.180.17
  • The values presented in this table were generated by a least squares (LS) fit of the average experimental fitness functions plotted in Fig 1. Parameter A has units of μg/ml and corresponds to the cytoplasmic drug concentration, [S]C, at which the normalized growth rate is half maximal. Parameter A can also roughly be interpreted as the apparent Kd of the drug to the ribosome in addition to all other intercellular drug interactions. Parameter B is the Hill coefficient that describes the shape of the dose–response curve in response to the antibiotic and is thus a highly complex lumped parameter that models essentially all aspects of cellular physiology as one variable.