Table 1. Overview of enriched protein complexes and identified biotinylated subunits
NTREnriched protein complexes and proteins of similar biological functionNumber of identified subunits (ES > 25)Biotinylated subunit
NTR‐BirA*BirA*‐NTR
IMA1Condensin ll complex5 (5)4 (0)SMC4, hCAP‐D3
H/ACA ribonucleoprotein complex5 (5)3 (0)DKC1
DNA replication complex GINS3 (3)1 (0)
Little elongation complex4 (4)4 (3)ELL, ICE1
Integrator complex8 (6)7 (0)INTS4, INTS10
Exosome complex11 (10)11 (0)EXOSC9, EXOSC10, RRP44
DNA‐directed RNA polymerase l9 (9)7 (5)
DNA‐directed RNA polymerase lll15 (14)11 (7)RPC3
Chromodomain‐helicase‐DNA‐binding protein6 (5)6 (1)CHD4, CHD7, CHD8
DNA mismatch repair protein5 (5)5 (2)MLH1, MSH6
Mediator of RNA polymerase II transcription19 (17)13 (3)MED1, MED27
MORC family CW‐type zinc finger protein3 (3)3 (1)
Negative elongation factor4 (4)4 (1)NELF‐A, NELF‐E
Transcription initiation factor TFIID13 (13)11 (11)TAF1, TAF3, TAF7
U6 snRNA‐associated Sm‐like protein7 (7)6 (6)
Putative polycomb group protein ASXL3 (3)2 (0)
IMA5Condensin ll complex4 (1)2 (0)SMC4
H/ACA ribonucleoprotein complex5 (5)5 (4)DKC1
Little elongation complex4 (3)4 (2)ELL
Integrator complex9 (8)8 (5)INTS4, INTS10, INTS11
Exosome complex10 (6)11 (1)EXOSC9, EXOSC10, RRP44
DNA‐directed RNA polymerase l9 (7)7 (5)RPAC2
DNA‐directed RNA polymerase lll13 (9)10 (5)RPAC2, RPC3
Chromodomain‐helicase‐DNA‐binding protein6 (5)5 (2)CHDL1, CHD3, CHD7, CHD8
DNA mismatch repair protein4 (3)5 (1)MLH1, MSH6
IMA6H/ACA ribonucleoprotein complex5 (4)DKC1
Little elongation complex4 (3)ICE1
IPO4Anaphase‐promoting complex9 (3)9 (4)APC3
AP2 adaptor complex5 (3)4 (1)
HAUS augmin‐like complex8 (8)8 (7)HAUS2*
Eukaryotic translation initiation factor 313 (9)13 (11)Subunit D, E, G, J
Activating signal cointegrator (ASC‐1)4 (4)4 (4)TRIP4, ASCC1
Histone chaperone ASF12 (2)2 (2)
Transforming acidic coiled‐coil‐containing protein3 (3)3 (3)TACC1, TACC3
IPO5Anaphase‐promoting complex8 (0)9 (9)
AP2 adaptor complex5 (0)4 (4)
HAUS augmin‐like complex8 (2)8 (7)
Eukaryotic translation initiation factor 312 (6)13 (13)Subunit A, D, E, G, J
WASH complex4 (2)4 (4)
IPO11Basic leucine zipper and W2 domain‐containing protein2 (2)BZW1, BZW2
IPO13Special AT‐rich sequence‐binding protein2 (2)SATB1
TNPO1Argonaute protein family2 (2)
Nuclear receptor coactivator6 (5)SRC2*
TNPO2Argonaute protein family3 (3)3 (3)
Nuclear receptor coactivator6 (6)6 (6)
CREB‐regulated transcription coactivator and binding protein4 (4)4 (4)CRTC1, CRTC2, CRTC3
Far upstream element‐binding protein3 (2)3 (3)FUBP1, FUBP2, FUBP3
CCAAT‐box‐binding transcription factor (CTF)3 (3)3 (2)
XPO1Little elongation complex4 (4)
Exosome complex10 (2)EXOSC10
XPO2DNA replication complex GINS3 (3)2 (1)
Heterogeneous nuclear ribonucleoprotein11 (0)21 (17)Protein A1, F, M, U, UL1
XPO7COP9 signalosome9 (9)CSN4
  • Proteins labeled with an asterisk were only identified in the ACN/TFA elution dataset.