Table 1. Promoter and terminator part characterization
Genetic context
Isolation (Cytometry)bCircuit (RNA‐seq)c
Promotersa1st2nd1st2nd
PTac‐PTet154858 ± 217 ± 5
PBAD1‐PTet24985242 ± 118283 ± 237
PBAD24926 ± 29
PBM3R1‐PAmtR10759 ± 455 ± 32
PSrpR‐PLitR418537 ± 2014 ± 4
PPhlF80323 ± 25
Terminators
L3S2P5541824 ± 6
L3S2P24212295 ± 176
L3S2P11384110 ± 89
ECK120029600374380 ± 98
ECK120033737391870 ± 344
L3S2P21505187 ± 127
  • a The strength of the left promoter is 1st; right is 2nd.

  • b Previously reported promoter strengths (in au/s) based on a fluorescent reporter (Nielsen et al, 2016), converted to au/s as described in text. Previously reported termination strengths (Chen et al, 2013).

  • c Average and standard deviations are calculated from three replicates performed on different days. For promoters, all states where the promoter is predicted to be on are included and the units are au/s. For double promoters, separate strengths for each promoter are calculated as described in the text. Median terminator strengths are calculated for states where the upstream gene is on. For terminators L3S2P24 and L3S2P11 in one replicate, the data for input state −/−/+ were excluded due to a mapping bias (Appendix Fig S1).