Table 1. Highly expressed enzymes for each step in the degradation of hemicellulose and cellulose
ProteinFunctionCAZyHemicelluloseCellulosePsortSignalp‐NN
Cphy2108Cell surface 1,4‐β‐d‐xylanaseGH10, CBM225737.9(S)563.8(S)Cell wall0.9
Cphy21051,4‐β‐d‐XylanaseGH113120.7(S)6007.9Extracellular0.97
Cphy15101,4‐β‐d‐XylanaseGH102074.5(S)29559.8(S)Extracellular0.9
Cphy06241,4‐β‐d‐XylanaseGH10, CBM221055.6(S)4767.9(S)Extracellular0.65
Cphy30101,4‐β‐d‐XylanaseGH10933.10.0Extracellular
Xylosaccharides to xylose
Cphy3009Non‐reducing end 1,4‐β‐xylosidaseGH35982.0542.6Cytoplasmic
Cphy3207Reducing end 1,4‐β‐xylosidaseGH82473.20.0Unknown
Remove hemicellulose side groups
Cphy3158α‐1,2‐GlucuronosidaseGH673508.982.4Cytoplasmic
Cphy3160β‐Glucuronidase, galactosidaseGH2813.31701.1Cytoplasmic
Cphy2632ArabinaseGH43703.40.0Cytoplasmic
Cphy2848α‐Glucuronidase, galactosidaseGH4701.1199.9(S)Cytoplasmic
Cphy3862Xylanase, carboxylesteraseGH10, CE15466.8(S)1511.7(S)Extracellular0.9
Cphy2730Acetyl xylan esteraseCE4155.4225.6Cytoplasmic
Cellulose
Hydrolysis of cellulose to cellodextrins
Cphy3368ExocellulaseGH48, CBM31911.7(S)9730.3(S)Extracellular0.65
Cphy3367Bifunctional endo, exocellulaseGH9, CBM32181.4(S)9277.7(S)Extracellular0.87
Cphy3202CellulaseGH5, CBM2, CBM461550.3(S)3485.3(S)Unknown0.81
Cphy2058CellulaseGH5424.5(S)1199.0(S)Unknown0.62
Cphy1163CellulaseGH5136.9(S)525.1Unknown0.82
Hydrolysis of cellodextrins
Cphy0220Non‐reducing end β‐glucosidaseGH3688.93469.6Cytoplasmic
Cphy1169Endo‐1,4‐β‐d‐glucanaseGH51112.0615.3Cytoplasmic
Phosphorolytic cleavage of cellodextrins to glucose‐1‐phosphate
Cphy3854Cellodextrin, cellobiose phosphorylaseGH94136.47602.4Membrane
Cphy0430Cellodextrin, cellobiose phosphorylaseGH94166.24421.4Membrane
Cphy1929Cellodextrin, cellobiose phosphorylaseGH94133.62743.0Membrane
Other highly expressed CAZy
Cphy1799ChitinaseGH18, CBM12175.3(S)43042.0Unknown0.77
Cphy1800ChitinaseGH18, CBM12101.9(S)31498.1(S)Extracellular0.8
Cphy0218α‐GlucosidaseGH31184.53366.0Cytoplasmic
Cphy1687Polysaccharide deacetylaseCE4, CBM360.02551.3(S)Extracellular0.858
Cphy1888Pectin lyasePL90.02334.6Extracellular0.72
Cphy1071β‐MannanaseGH26, CBM3, CBM35764.5(S)1664.3(S)Extracellular0.68
Cphy1652ChitinaseGH18, CBM500.01329.0(S)Cytoplasmic
Cphy2128β‐MannanaseGH26, CBM3, CBM35153.7(S)956.0(S)Extracellular0.78
Cphy1943ChitinaseGH19625.6(S)875.4Unknown0.77
  • Protein ID, putative function, CAZy category, APEX expression, localization (PsortB v2.0), and significant signal peptide predictions (SignalP3.0‐NN D‐value>0.45) are shown. APEX values followed by (S) are supernatant proteins.

  • Hemicellulose

  • Xylan backbone to xylosaccharides