Table 2. Knockout efficiencya
Percent correctbORFsEssentiality scorec
<−1−1 to +1⩾+1
10019461916300
87.5729719100
75499487120
62.531630790
5021921180
37.511611240
25160149110
12.51010
0302088214
Total42883901173214
  • ORF=open‐reading frame.

  • a Data are in given Supplementary Table 3.

  • b Percent of the four or eight KmR colonies shown to have the correct structure by PCR as described in text is given.

  • c The number of ORFs with different essentiality scores is given. Scores less than −1 or greater than +1 mean that the gene is nonessential (<−1) or essential (>+1) with no inconsistency with previous studies. Scores between −1 and +1 mean some inconsistency exists.