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  • Molecular Systems Biology: 14 (7)

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Article

Thermal proteome profiling in bacteria: probing protein state in vivo

View ORCID ProfileAndré Mateus, Jacob Bobonis, View ORCID ProfileNils Kurzawa, Frank Stein, Dominic Helm, Johannes Hevler, View ORCID ProfileAthanasios Typas, View ORCID ProfileMikhail M Savitski
DOI 10.15252/msb.20188242 | Published online 06.07.2018
Molecular Systems Biology (2018) 14, e8242
André Mateus
Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
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Jacob Bobonis
Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, GermanyFaculty of Biosciences, Heidelberg University, Heidelberg, Germany
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Nils Kurzawa
Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, GermanyFaculty of Biosciences, Heidelberg University, Heidelberg, Germany
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Frank Stein
Proteomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany
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Dominic Helm
Proteomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany
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Johannes Hevler
Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
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Athanasios Typas
Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
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Mikhail M Savitski
Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
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Author Affiliations

  1. André Mateus1,
  2. Jacob Bobonis1,2,
  3. Nils Kurzawa1,2,
  4. Frank Stein3,
  5. Dominic Helm3,
  6. Johannes Hevler1,
  7. Athanasios Typas (typas{at}embl.de)*,1 and
  8. Mikhail M Savitski (mikhail.savitski{at}embl.de)*,1
  1. 1Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
  2. 2Faculty of Biosciences, Heidelberg University, Heidelberg, Germany
  3. 3Proteomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany
  1. ↵* Corresponding author. Tel: +49 6221 387 8156; E‐mail: typas{at}embl.de
    Corresponding author. Tel: +49 6221 387 8560; E‐mail: mikhail.savitski{at}embl.de
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  • Figure 1.
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    Figure 1. Thermal proteome profiling in Escherichia coli

    1. Thermal proteome profiling protocol overview. After cells are grown to a specified optical density (OD578), aliquots are heated to a range of temperatures, lysed, and the remaining soluble fraction of the proteome is collected. Mass spectrometry‐based proteomics (using tandem mass tags, TMT) is then used to quantify the amount of protein at each condition, and melting curves are plotted for each protein.

    2. Melting curves for E. coli proteins. The average melting curve for each cellular compartment is shown.

    3. Distribution of melting temperatures (Tm) of the E. coli and the human proteomes.

    4. Distribution of melting temperatures (Tm) of the E. coli and the human proteomes according to selected gene ontology terms. Line represents the median, box represents the interquartile range, and whiskers of the box plots represent the 5th and 95th percentiles.

    5. Distribution of melting temperatures (Tm) of the E. coli proteome according to their cellular compartment. Box plots are plotted as panel (D).

    6. Correlation of melting points in lysate determined by TPP (this study) with melting points determined by limited proteolysis coupled to mass spectrometry (Leuenberger et al, 2017). For the results from Leuenberger et al (2017), the median melting point of the reported peptides for each protein was used. Only proteins with at least two identified peptides were compared. Red dots represent ribosomal proteins, which generally appear less thermostable in TPP.

    7. Correlation of melting points in lysate determined by TPP upon addition of 10 mM MgCl2 (this study) with melting points determined by limited proteolysis coupled to mass spectrometry (Leuenberger et al, 2017). For the results from Leuenberger et al (2017), the median melting point of the reported peptides for each protein was used. Only proteins with at least two identified peptides were compared. Red dots represent ribosomal proteins.

  • Figure EV1.
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    Figure EV1. Melting behavior of proteins identified in the Escherichia coli meltome and their properties

    • A. Reproducibility of identified proteins in each replicate of E. coli meltome analysis.

    • B. Overlap of identified proteins with previously published proteomics datasets obtained from E. coli.

    • C. Distribution of differences between protein abundance after being extracted with NP‐40 or with SDS.

    • D, E Correlation of melting point with (D) protein abundance (r = 0.06, P = 0.015, as measured by the top3 intensity corresponding to the lowest temperature) and (E) molecular weight (r = −0.08, P = 0.0009).

    • F. Correlation of melting point in living cells with melting point in lysate—both from TPP (r = 0.82, P < 0.0001).

    • G. Melting curves for E. coli outer membrane proteins. The average melting curve for each class of outer membrane proteins is shown.

    • H. Distribution of melting temperatures (Tm) of the E. coli proteome according to their cellular compartment.

    • I. Fraction of proteins with Tm > 87°C (the highest temperature tested) in each cellular compartment.

  • Figure 2.
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    Figure 2. Impact of growth phase on the Escherichia coli meltome and proteome

    1. Melting temperatures (Tm) of proteins in exponential and transition to stationary growth phases. Proteins highlighted in orange indicate significantly different melting behavior.

    2. Protein abundance in exponential and transition to stationary growth phases, as measured by the top3 intensity corresponding to the lowest temperature (see “Materials and Methods”). Proteins highlighted in orange indicate significantly different levels. Proteins were considered not detectable (n.d.) in one condition, if absent in three replicates in that condition, but detectable by at least three unique peptides in at least two replicates in the other condition.

    3. Respiratory activity in exponential and stationary cells determined as the conversion of triphenyltetrazolium chloride to triphenylformazan during the same time and normalized by OD (˜number of cells). n = 3; error bars represent standard deviation; **P < 0.01, Student's t‐test.

  • Figure 3.
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    Figure 3. Melting behavior of protein complexes

    • A. Melting behavior of protein complexes from human and Escherichia coli was measured by the average Euclidean distance between the melting curves of proteins from each complex. Line represents the median, box represents the interquartile range, and whiskers of the box plots represent the 10th and 90th percentiles. Pie charts represent the fraction of protein complexes that melt coherently (compared with a distribution of 10,000 random complexes; P < 0.05).

    • B. Comparison of the melting behavior of protein complexes located in a single cellular compartment or in multiple compartments. Line represents the median, box represents the interquartile range, and whiskers of the box plots represent the 10th and 90th percentiles. Pie charts represent the fraction of protein complexes that melt coherently (compared with a distribution of 10,000 random complexes; P < 0.05).

    • C. Schematic representation of complexes located in a single cellular compartment or in multiple compartments.

    • D–H Melting curves for examples of non‐co‐melting complexes located in the same cellular compartment: (D) ClpP protease complex, (E) Ruv DNA repair complex, (F) Uvr DNA repair complex, (G) Suf Fe‐S biogenesis complex, and (H) Bam outer membrane porin assembly complex. P indicates the probability that the complex melts coherently (compared with a distribution of 10,000 random complexes).

  • Figure EV2.
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    Figure EV2. Melting behavior of protein complexes

    Euclidean distance between all the pairs of melting curves of proteins from each complex—collected from EcoCyc v.21.1 (https://ecocyc.org/; Keseler et al, 2017). Only proteins detected with at least two unique peptides in at least two replicates are shown. Node color represents protein location, and edge color represents the average Euclidean distance between the melting curves of complex members.

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    Figure 4. Effect of gene knockout on protein thermostability and abundance

    • A. Two‐dimensional thermal proteome profiling (2D‐TPP) protocol overview. Wild‐type (WT) and tolC knockout strain (ΔtolC) were grown and prepared in a similar manner to what is described in Fig 1A. For each protein, abundance and stability scores were calculated.

    • B, C Volcano plots for abundance (B) and stability (C) scores for each identified protein in ΔtolC compared to WT (TolC signal is detected at noise level in the ΔtolC strain, due to its presence in WT and TMT quantification rarely producing missing values). Proteins highlighted in orange show significant changes [false discovery rate (FDR) P < 0.05 and absolute score > 10].

    • D. Proposed mechanism for abundance and stability hits of ΔtolC.

    • E. Schematic representation of TolC complexes and the stability scores of their members. YbhG is a member of a putative efflux transporter. *False discovery rate (FDR) P < 0.05 and absolute score > 10. n.d. not detected.

    • F, G Cell growth (as measured by OD595) after 8 h in the presence of azithromycin (F) or aztreonam (G) in WT, ΔtolC, ΔompF, and ΔmicFΔtolC cells (n = 4; error bars represent standard deviation).

    • H. Target engagement affinity of aztreonam in WT and ΔtolC cells, measured by thermal proteome profiling–compound concentration range (TPP‐CCR). Stabilization of the main known target of aztreonam (FtsI) is shown.

  • Figure EV3.
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    Figure EV3. Effects of ΔtolC on protein thermostability and abundance

    • A, B Interaction network of TolC color‐coded by the (A) abundance score or (B) stability score. Network was obtained from STRING database by querying only the statistically significant hits (in both abundance and stability) and their interactions with a confidence score of > 0.4.

    • C. Growth of ΔsurAΔtolC in the absence or presence of 1 mM MgSO4 and 0.1 mM CaCl2 in LB medium containing 4 mM sodium citrate with or without 30 μg/ml kanamycin. Viability was determined by spotting serial dilutions (100−10−6) of overnight cultures.

    • D. Cell growth (as measured by OD595) in LB supplemented with 600 mM NaCl of WT, ΔtolC, ΔsurA, and ΔsurAΔtolC from a starting culture at OD595 = 0.2.

    • E. Growth of WT, ΔtolC, ΔsurA, and ΔsurAΔtolC in increasing concentrations of NaCl in LB. Viability was determined by spotting serial dilutions (100–10−6) of overnight cultures.

    • F. Cell growth (as measured by OD595) after 8 h in the presence of aztreonam in WT, ΔtolC, ΔompF and ΔompF ΔtolC cells (n = 4; error bars represent standard deviation).

    • G. Cell growth (as measured by OD595) after 8 h in the presence of aztreonam in WT, ΔtolC, ΔmicF, and ΔmicF ΔtolC cells (n = 4; error bars represent standard deviation).

    • H. Target engagement affinity of aztreonam in WT and ΔtolC cells, measured by thermal proteome profiling compound concentration range (TPP‐CCR). Stabilization of the secondary known target of aztreonam (MrcA) is shown.

  • Figure 5.
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    Figure 5. Target identification of ampicillin and ciprofloxacin

    1. 2D‐TPP protocol overview. After treatment with different concentrations of antibiotics, the cells were prepared in a similar manner to what is described in Fig 1A. For each protein and temperature, the signal intensity was normalized to the vehicle control.

    2. Heatmaps for targets of ampicillin in living cells, with coloring according to panel (A). *FDR controlled at 1% using a bootstrapped permutation approach.

    3. Number of stabilized or destabilized proteins in lysate and living cells after treatment with ampicillin.

    4. Example of top gene ontology terms enriched in proteins affected in living cells after treatment with ampicillin.

    5. Heatmaps for targets of ciprofloxacin in living cells, with coloring according to what is described in panel (A). *FDR controlled at 1% using a bootstrapped permutation approach.

    6. Schematic representation of SOS response. LexA binds to promoters of SOS response genes and represses their transcription. Treatment with ciprofloxacin induces single‐ and double‐stranded DNA breaks that recruit RecA to DNA, causing auto‐cleavage of LexA and expression of SOS response genes (e.g., yebG; Andersson & Hughes, 2014).

  • Figure EV4.
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    Figure EV4. Identification of direct targets of ampicillin and ciprofloxacin

    • A, B Heatmaps of effects on thermostability of proteins after treatment with (A) ampicillin and (B) ciprofloxacin in lysate, with coloring according to what is described in Fig 5A. *FDR controlled at 1% using a bootstrapped permutation approach.

Supplementary Materials

  • Figures
  • Expanded View Figures PDF [msb188242-sup-0001-EVFigs.pdf]

  • Dataset EV1 [msb188242-sup-0002-DatasetEV1.xlsx]

  • Dataset EV2 [msb188242-sup-0003-DatasetEV2.xlsx]

  • Dataset EV3 [msb188242-sup-0004-DatasetEV3.xlsx]

  • Dataset EV4 [msb188242-sup-0005-DatasetEV4.xlsx]

  • Dataset EV5 [msb188242-sup-0006-DatasetEV5.xlsx]

  • Dataset EV6 [msb188242-sup-0007-DatasetEV6.xlsx]

  • Dataset EV7 [msb188242-sup-0008-DatasetEV7.xlsx]

  • Dataset EV8 [msb188242-sup-0009-DatasetEV8.xlsx]

  • Dataset EV9 [msb188242-sup-0010-DatasetEV9.xlsx]

  • Dataset EV10 [msb188242-sup-0011-DatasetEV10.xlsx]

  • Dataset EV11 [msb188242-sup-0012-DatasetEV11.xlsx]

  • Dataset EV12 [msb188242-sup-0013-DatasetEV12.xlsx]

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Volume 14, Issue 7
01 July 2018
Molecular Systems Biology: 14 (7)
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