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LuTHy: a double‐readout bioluminescence‐based two‐hybrid technology for quantitative mapping of protein–protein interactions in mammalian cells

View ORCID ProfilePhilipp Trepte, Sabrina Kruse, Simona Kostova, Sheila Hoffmann, Alexander Buntru, Anne Tempelmeier, Christopher Secker, View ORCID ProfileLisa Diez, Aline Schulz, Konrad Klockmeier, Martina Zenkner, Sabrina Golusik, Kirstin Rau, Sigrid Schnoegl, Craig C Garner, View ORCID ProfileErich E Wanker
DOI 10.15252/msb.20178071 | Published online 11.07.2018
Molecular Systems Biology (2018) 14, e8071
Philipp Trepte
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Sabrina Kruse
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Simona Kostova
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Sheila Hoffmann
Synaptopathy, German Center for Neurodegenerative Diseases, Berlin, Germany
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Alexander Buntru
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Anne Tempelmeier
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Christopher Secker
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, GermanyDepartment of Neurology, Charité Universitätsmedizin Berlin, Berlin, Germany
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Lisa Diez
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Aline Schulz
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Konrad Klockmeier
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Martina Zenkner
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Sabrina Golusik
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Kirstin Rau
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Sigrid Schnoegl
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Craig C Garner
Synaptopathy, German Center for Neurodegenerative Diseases, Berlin, Germany
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Erich E Wanker
Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
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Author Affiliations

  1. Philipp Trepte1,
  2. Sabrina Kruse1,
  3. Simona Kostova1,
  4. Sheila Hoffmann2,
  5. Alexander Buntru1,
  6. Anne Tempelmeier1,
  7. Christopher Secker1,3,
  8. Lisa Diez1,
  9. Aline Schulz1,
  10. Konrad Klockmeier1,
  11. Martina Zenkner1,
  12. Sabrina Golusik1,
  13. Kirstin Rau1,
  14. Sigrid Schnoegl1,
  15. Craig C Garner2 and
  16. Erich E Wanker (ewanker{at}mdc-berlin.de)*,1
  1. 1Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
  2. 2Synaptopathy, German Center for Neurodegenerative Diseases, Berlin, Germany
  3. 3Department of Neurology, Charité Universitätsmedizin Berlin, Berlin, Germany
  1. ↵*Corresponding author. Tel: +49 30 9406 2157; E‐mail: ewanker{at}mdc-berlin.de
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Article Information

vol. 14 article no. e8071

DOI 
https://doi.org/10.15252/msb.20178071
PubMed 
29997244
Print ISSN 
1744-4292
Online ISSN 
1744-4292
History 
  • Published online July 11, 2018.
  • Published in print July 1, 2018.
Review Process File

Author Information

  1. Philipp Trepte1,
  2. Sabrina Kruse1,
  3. Simona Kostova1,
  4. Sheila Hoffmann2,
  5. Alexander Buntru1,
  6. Anne Tempelmeier1,
  7. Christopher Secker1,3,
  8. Lisa Diez1,
  9. Aline Schulz1,
  10. Konrad Klockmeier1,
  11. Martina Zenkner1,
  12. Sabrina Golusik1,
  13. Kirstin Rau1,
  14. Sigrid Schnoegl1,
  15. Craig C Garner2 and
  16. Erich E Wanker (ewanker{at}mdc-berlin.de)*,1
  1. 1Neuroproteomics, Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, Berlin, Germany
  2. 2Synaptopathy, German Center for Neurodegenerative Diseases, Berlin, Germany
  3. 3Department of Neurology, Charité Universitätsmedizin Berlin, Berlin, Germany
  1. ↵*Corresponding author. Tel: +49 30 9406 2157; E‐mail: ewanker{at}mdc-berlin.de
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Volume 14, Issue 7
01 July 2018
Molecular Systems Biology: 14 (7)
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