Tandem fluorescent protein timers are used to distinguish between models of morphogen gradient formation.

The spatial distribution of protein age provides insights into the underlying kinetics and indicates an effective Bicoid diffusion constant of 3–4 μm²/s.

Bicoid protein degradation is likely playing a critical role in forming the Bicoid morphogen gradient and the Bicoid half‐life is estimated to be around 25 min.

Tissue‐scale coordination in murine dermis is driven by the interdependence of cell proliferation and ECM deposition.

The tissue architecture is set by a negative feedback loop between ECM deposition/remodelling and proliferation.

Fibroblast lineages lose segregation with age.

Fibroblast migration is the critical discriminator between dermal development and wound healing.

Cell lines are developed with doxycycline‐inducible expression of specific miRNAs.

Stochastic variation in single‐cell gene expression can be used to infer biochemical parameters of miRNA‐target interaction.

The inferred parameters indicate that in this experimental system, endogenous targets are only marginally affected by the expression of a competing RNA.

These analyses further reveal that at intermediate expression levels, where targets respond ultrasensitively to the miRNA, the correlation in target expression increases 2–3 fold.